Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Cromosomas politénicos |

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Preparación de los cromosomas politénicos de Drosophila Calabazas para el Etiquetado de anticuerpos

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Warm to disperse the Triton X That is the volume I prepare each time, usually enough for two or three prep. If you have any question you can send me another e-mail.

This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect?

Is it the PFA? My thought is that they may be getting flushed out during my transfers. Do you have any advice?

You are right about the Cromosomsa. If you leave the glands in too long they will fall apart. The trick is to leave them in just long enough. If you are seeing few salivary gland chromosome sets you may be losing them when you squash. Politeniocs you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present.

Infográfico: Cromosomas politénicos ~ Ciber-Genética

Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. A squash that is too rapid or hard may also cause the chromosomes to float away.

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Your institution must subscribe to JoVE’s Biology section to access this content. Fill out the form below to receive a free trial or learn more about access: Caliente para dispersar a la Triton X y su uso dentro de una hora. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas. Repita los pasos 4.

Lugar pesos g en el cubreobjetos de las diapositivas.

Examinar el portaobjetos una vromosomas que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos. The dissection forceps we use were mis-identified: A subscription to J o VE is required to view this article. You will only be able to see the first 20 seconds. Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes.

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